Laser scanning confocal microscope Leica SP8 X FLIM

Contacts

+385 1 457 1219

Confocal laser scanning microscope is a light microscope used for monitoring and recording of living and inanimate microscopic specimens using fluorescence and reflected light. In this method, the specimen is illuminated point by point, and reemitted or reflected light is detected and recorded to build an image in computer memory. The main advantage of confocal microscopy vs. wide-field microscopy is its capability for optical sectioning, i.e. obtaining a sharp image of a thin section of a thick specimen. Confocal microscopy has a wide scope of applications similar to classical light microscopy. Our instrument is based on an inverse microscope.

General instrument information

Short name

New confocal

Inventory number

0042863

Characteristics

Model

Leica TCS SP8 X FLIM

Manufacturer

Leica Microsystems

Operating and measuring scope

Fluorescence and reflection microscopy, FLIM

Principle of work

confocal fluorescence microscopy

Detailed technical characteristics

Five discrete excitation laser lines: 405, 458, 488, 496 and 514 nm, and the supercontinuum excitation range from 470 to 700 nm. Laser intensity of 8 excitation lines can be adjusted continuously and independently. Four detection channels. Spectral detection system, AOBS, classic and resonant scanner, x, y, z, t and double lambda imaging modes. 12 bit dynamics, FRAP, FRET, FLIM.;Confocal laser scanning microscope is a light microscope used for monitoring and recording of living and inanimate microscopic specimens using fluorescence and reflected light. In this method, the specimen is illuminated point by point, and reemitted or reflected light is detected and recorded to build an image in computer memory. The main advantage of confocal microscopy vs. wide-field microscopy is its capability for optical sectioning, i.e. obtaining a sharp image of a thin section of a thick specimen. Confocal microscopy has a wide scope of applications similar to classical light microscopy. Our instrument is based on an inverse microscope.

Additional equipment

A system for maintenance of controlled conditions, temperature, atmosphere and humidity, in the specimen chamber. Programme for image visualization and analysis Huygens Professional.

Purchase price

5174800 HRK

Portability

Remote work

Location

Address

Bijenička 54

Room

5. krilo / suteren / 014B

Terms of use

Usage method

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